Here, data are presented that mammalian cell replication of DNAs containing these lesions was ∼97% accurate. To identify DNA polymerases that may function in TLS past ICLs, oligodeoxynucleotides were synthesized containing site-specific ICLs in which the linkage was between N 2-guanines, similar to cross-links formed by mitomycin C and enals. Although there exists compelling genetic evidence for a homologous recombination-independent pathway for repair of interstrand cross-links (ICLs) involving translesion synthesis (TLS), biochemical support for this model is lacking.
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